Submission S00038 (William Lees)
Submission Details
| Submission ID | S00038 |
| Submission Date | 2023-01-24 00:00:00 |
| Submission Status | reviewing |
| Submitter | William Lees |
| Submitter Address | Birkbeck College, University of London, Malet Street, London |
| Species | Homo sapiens |
| Ethnicity | UN |
Inferred Sequences
The inferred novel alleles from each genotype that are submitted for review. This table lists all inferences put forward by the submitter. Where IARC has affirmed a sequence based on an inference, the corresponding sequence record will be listed in the Published column. Inferences for which no published sequence is shown have not been affirmed.
No Items
Genotypes
Each genotype that has been inferred, along with the descriptive name of the inference tool and settings that were used.
Acknowledgements
Individuals who should be acknowledged as contributing to the inferences listed in this submission.
No Items
Repertoire Details
Details of the repertoire from which the inferences are based. This corresponds, for example, to an NIH Project or an ENA study.
| Repository | ENA |
| Accession Number | PRJEB26509 |
| Project/Study Title | Naive B-cell receptor heavy chain repertoire of celiac patients and healthy controls |
| Dataset URL | https://www.ebi.ac.uk/ena/browser/api/xml/PRJEB26509 |
| MiAIRR Compliant? | No |
| MiAIRR URL | |
| Sequencing Platform | Illumina MiSeq |
| Read Length | 300x300 |
| Primers Overlapping? | No |
Repertoire Publications
Publications associated with this study.
| PubMed ID | Title | Authors |
|---|---|---|
| 30733445 | Mosaic deletion patterns of the human antibody heavy chain gene locus shown by Bayesian haplotyping. | Gidoni M, Snir O, Peres A, Polak P, Lindeman I, Mikocziova I, Sarna VK, Lundin KEA, Clouser C, Vigneault F, Collins AM, Sollid LM, Yaari G |
PCR Primers
Sequences of the PCR primers used in the study.
3' Primers
As the protocol used was 5’ RACE, there is only one primer set.
| Primer Name | Primer Sequence |
|---|---|
| Human-IGHM | GAATTCTCACAGGAGACGAGG |
| Human-IGHD | TGTCTGCACCCTGATATGATGG |
| Human-IGHA | GGGTGCTGYMGAGGCTCAG |
| Human-IGHE | TTGCAGCAGCGGGTCAAGG |
| Human-IGHG | CCAGGGGGAAGACSGATG |
| Human-IGK | GACAGATGGTGCAGCCACAG |
| Human-IGL | AGGGYGGGAACAGAGTGAC |