Details

SpeciesHuman
Sequence NameIGHV2-70*i01
IUIS NameIGHV2-70*20
Seq IDOGRDB:RRB5
Affirmation Level1
Full Sequence
Coding Sequence
FunctionalTrue
Inference TypeRearranged Only
Alternative namesIGHV2-70*20
Paralogs

Evidence

The table below lists the submissions, and the inferences within them, on which this published sequence is based. The list may be short when the sequence is first published, containing, for example, reference to just a single submission, but is expected to grow over time as additional inferences are submitted.

'Sequence Match' indicates whether the inference exactly matches the sequence, and clicking on the tick or cross will provide an alignment. Mismatches may be caused, for example, by the inclusion of leader sequences, or nucleotides in the inference which do not in IARC's opinion have sufficient evidence for inclusion in the sequence.

Submission IDAccession NoSubject IDGenotype NameSequence NameSequence Match
S00010 MK471379B12B12IGHV2-70*01_S6619

Supporting Observations

Sequences in other published genotypes which have been identified by IARC and support the inference:

No Items

Observations in VDJbase

Inferred sequences in VDJbase that match this sequence:

No Items

Un-rearranged Observations

Un-rearranged sequence observations that support this sequence:

No Items

Additional Information

Sequence IDA00044
Curator addressDept. of Immunotechnology, Lund University, Medicon Village building 406, S-22381 Lund, Sweden
Version1
Release Date2021-05-18
Release Notes

IGHV2-70*i01 was elevated to Level 1 on May 18th 2021 at IARC Meeting 69, in recognition of the entry of this sequence into the IMGT germline gene database, as a result of submission of the sequence to IMGT by processes external to the IARC.

LocusIGH
Sequence TypeV
Gene Subgroup2
Gene Designation70
Allele Designationi01

Acknowledgements

Individuals acknowledged as contributing to this sequence:

No Items

Notes

Notes are added by IARC reviewers.

Assessed by IARC on November 18, 2019 during meeting 45.

The committee considered IGHV2-70*01_S6619 (A124G) of Genotype B12 of submission S00010, which was previously evaluated as IGHV2-70*01_S4660 of submission S00004 at Meeting 8. It was affirmed as a Level 0 sequence at that time.

The sequence was seen in just 0.05% of all unmutated rearrangements, with 296 sequences including 93 perfect matches to the inferred allele. There was abundant variation in the CDR3 regions of the aligned sequences. Two other alleles were present in the genotype, and it is possible that one of these three sequences is an IGHV2-70D sequence. Haplotype data is supportive of the inference, but in light of the very low frequency of rearrangements seen and the complicated haplotype of this subject, it was affirmed as a Level 0 sequence. The sequence was affirmed up to and including nucleotide 319 even though alleles of IGHV2-70/IGHV2-70D in general extend up to and including base 322. However, the nucleotide composition of the subsequent three bases of genes associated with this inferred allele did not support their inference. It is however likely that three additional 3’ nucleotides are present in the sequence, and this is indicated in IARC and OGRDB publications by three dots at the end of the sequence.

At the IARC Meeting 68, April 26th 2021, it was agreed that sequences affirmed by the IARC as Level 0 sequences, that subsequently have entered the IMGT germline gene database by other means, should be elevated to Level 1.

At the IARC Meeting 69, May 18th 2021, IGHV2-70*i01 was elevated to Level 1. This sequence was incorporated into the IMGT germline gene database as a consequence of the IMGT-NC Report #2020-2-1008 (October 8th, 2020). The sequence was submitted to IMGT by Abdullah Gamal Dorgham, William Sinclair Gibson, Oscar Rodriguez and Corey Watson. The sequence recorded in OGRDB remains the sequence that was affirmed as the original IGHV2-70*i01 sequence. The sequence that is now recognized as IGHV2-70*20 includes the final three nucleotides of the sequence – TAC.

Attachments

Attachment File Name
 

History

History logs the times and reasons for the publication of each version of this sequence.

Andrew Collins
2019-12-12 22:50:56
Version 1 published

Submission published by IARC on Dec 13, 2019.

Andrew Collins
2021-05-18 13:27:45
Version 2 published

IGHV2-70*i01 was elevated to Level 1 on May 18th 2021 at IARC Meeting 69, in recognition of the entry of this sequence into the IMGT germline gene database, as a result of submission of the sequence to IMGT by processes external to the IARC.

Mats Ohlin
2021-05-18 23:41:47
IMGT Name updated to IGHV2-70*20

IMGT Name updated.

Mats Ohlin
2021-05-18 23:41:53
IMGT Name updated to IGHV2-70*20

IMGT Name updated.

Versions

All published versions of this sequence.

Sequence NameIMGT NameAffirmation LevelSeq IDVersionDate
IGHV2-70*i010OGRDB:RRB512019-12-12
IGHV2-70*i01IGHV2-70*201OGRDB:RRB512021-05-18