Details


Species	Human
Species subgroup
Subgroup type
Sequence Name	IGHV2-70*20
IUIS Name	IGHV2-70*20
Alternative names	IGHV2-70*i01
Affirmation Level	1
Full Sequence
Coding Sequence
Functionality	ORF
Inference Type	Rearranged Only
Mapped	True
Paralogs
Paralog Rep	False

Un-rearranged Observations

Un-rearranged sequence observations that support this sequence:

Accession	Type	Repository	Start	End
MK471379	Inferred	GenBank	1	298

Observations in AIRR-seq Repertoires

Click here to review supporting data in VDJbase.

Clicking the link will take you to VDJbase. Open in a new tab if you want to keep this page open. In VDJbase, click on the count in the Apperances column to see a list of samples in which the sequence was found.

CDR delineation


CDR1 Start	76
CDR1 End	105
CDR2 Start	157
CDR2 End	177
CDR3 Start	292

Extension


3' Extension	TAC
3' start
3' end

Additional Information


Sequence ID	A00044
Curator	William Lees
Curator address	Birkbeck College, University of London, Malet Street, London
Version	2
Release Date	2023-07-10
Release Notes	Bulk upload of sequences for the AIRR-C Human IG germline sets
Locus	IGH
Sequence Type	V
Gene Subgroup	2
Gene Designation	70
Allele Designation	20

Acknowledgements

Individuals acknowledged as contributing to this sequence:

No Items

Notes

Notes are added by IARC reviewers.


Assessed by IARC on November 18, 2019 during meeting 45. The committee considered IGHV2-7001_S6619 (A124G) of Genotype B12 of submission S00010, which was previously evaluated as IGHV2-7001_S4660 of submission S00004 at Meeting 8. It was affirmed as a Level 0 sequence at that time. The sequence was seen in just 0.05% of all unmutated rearrangements, with 296 sequences including 93 perfect matches to the inferred allele. There was abundant variation in the CDR3 regions of the aligned sequences. Two other alleles were present in the genotype, and it is possible that one of these three sequences is an IGHV2-70D sequence. Haplotype data is supportive of the inference, but in light of the very low frequency of rearrangements seen and the complicated haplotype of this subject, it was affirmed as a Level 0 sequence. The sequence was affirmed up to and including nucleotide 319 even though alleles of IGHV2-70/IGHV2-70D in general extend up to and including base 322. However, the nucleotide composition of the subsequent three bases of genes associated with this inferred allele did not support their inference. It is however likely that three additional 3’ nucleotides are present in the sequence, and this is indicated in IARC and OGRDB publications by three dots at the end of the sequence. At the IARC Meeting 68, April 26th 2021, it was agreed that sequences affirmed by the IARC as Level 0 sequences, that subsequently have entered the IMGT germline gene database by other means, should be elevated to Level 1. At the IARC Meeting 69, May 18th 2021, IGHV2-70i01 was elevated to Level 1. This sequence was incorporated into the IMGT germline gene database as a consequence of the IMGT-NC Report #2020-2-1008 (October 8th, 2020). The sequence was submitted to IMGT by Abdullah Gamal Dorgham, William Sinclair Gibson, Oscar Rodriguez and Corey Watson. The sequence recorded in OGRDB remains the sequence that was affirmed as the original IGHV2-70i01 sequence. The sequence that is now recognized as IGHV2-70*20 includes the final three nucleotides of the sequence – TAC. Additional information for this sequence was imported into OGRDB via bulk update with the following notes: Sequence annotation is based on Genbank sample MK471379 Mapped by IARC haplotype

Assessed by IARC on November 18, 2019 during meeting 45.

The committee considered IGHV2-70*01_S6619 (A124G) of Genotype B12 of submission S00010, which was previously evaluated as IGHV2-70*01_S4660 of submission S00004 at Meeting 8. It was affirmed as a Level 0 sequence at that time.

The sequence was seen in just 0.05% of all unmutated rearrangements, with 296 sequences including 93 perfect matches to the inferred allele. There was abundant variation in the CDR3 regions of the aligned sequences. Two other alleles were present in the genotype, and it is possible that one of these three sequences is an IGHV2-70D sequence. Haplotype data is supportive of the inference, but in light of the very low frequency of rearrangements seen and the complicated haplotype of this subject, it was affirmed as a Level 0 sequence. The sequence was affirmed up to and including nucleotide 319 even though alleles of IGHV2-70/IGHV2-70D in general extend up to and including base 322. However, the nucleotide composition of the subsequent three bases of genes associated with this inferred allele did not support their inference. It is however likely that three additional 3’ nucleotides are present in the sequence, and this is indicated in IARC and OGRDB publications by three dots at the end of the sequence.

At the IARC Meeting 68, April 26th 2021, it was agreed that sequences affirmed by the IARC as Level 0 sequences, that subsequently have entered the IMGT germline gene database by other means, should be elevated to Level 1.

At the IARC Meeting 69, May 18th 2021, IGHV2-70*i01 was elevated to Level 1. This sequence was incorporated into the IMGT germline gene database as a consequence of the IMGT-NC Report #2020-2-1008 (October 8th, 2020). The sequence was submitted to IMGT by Abdullah Gamal Dorgham, William Sinclair Gibson, Oscar Rodriguez and Corey Watson. The sequence recorded in OGRDB remains the sequence that was affirmed as the original IGHV2-70*i01 sequence. The sequence that is now recognized as IGHV2-70*20 includes the final three nucleotides of the sequence – TAC.

Additional information for this sequence was imported into OGRDB via bulk update with the following notes:
Sequence annotation is based on Genbank sample MK471379
Mapped by IARC haplotype

Attachments

No Items

History

History logs the times and reasons for the publication of each version of this sequence.


Andrew Collins 2019-12-12 22:50:56	Version 1 published Submission published by IARC on Dec 13, 2019.


Andrew Collins 2021-05-18 13:27:45	Version 2 published IGHV2-70*i01 was elevated to Level 1 on May 18th 2021 at IARC Meeting 69, in recognition of the entry of this sequence into the IMGT germline gene database, as a result of submission of the sequence to IMGT by processes external to the IARC.


Mats Ohlin 2021-05-18 23:41:47	*IMGT Name updated to IGHV2-7020** IMGT Name updated.


Mats Ohlin 2021-05-18 23:41:53	*IMGT Name updated to IGHV2-7020** IMGT Name updated.


William Lees 2023-07-10 11:24:36	Version 2 published Bulk upload of sequences for the AIRR-C Human IG germline sets

Changes from previous version

	v1	v2
Curator		William Lees
Curator address	Dept. of Immunotechnology, Lund University, Medicon Village building 406, S-22381 Lund, Sweden	Birkbeck College, University of London, Malet Street, London
IUIS Name		IGHV2-70*20
Sequence Name	IGHV2-70*i01	IGHV2-70*20
Alternative names		IGHV2-70*i01
Mapped		True
Functionality	F	ORF
Affirmation Level	0	1
Species subgroup
Subgroup type
Allele Designation	i01	20
Full Sequence
Paralog Rep		False
Extension?	False	True
3' Extension		TAC
5' Extension
curational_tags		likely_truncated
Notes		changed

Versions

All published versions of this sequence.

Sequence Name	IMGT Name	Alternative names	Inference Type	Affirmation Level	Gene start	Gene end	CDR1 Start	CDR1 End	CDR2 Start	CDR2 End	CDR3 Start	Version	Date
IGHV2-70*i01			Rearranged Only	0	1	298	76	105	157	177	292	1	2019-12-12
IGHV2-70*i01	IGHV2-70*20	IGHV2-70*20	Rearranged Only	1	1	298	76	105	157	177	292	1	2021-05-18
IGHV2-70*20	IGHV2-70*20	IGHV2-70*i01	Rearranged Only	1	1	298	76	105	157	177	292	2	2023-07-10