Assessed by IARC on July 7, 2020 during meeting 57.

The committee considered IGLV2-14*03x (a 5’ extension of IGLV2-14*03) of submission S00028.

The sequence was seen in 6.08% of all unmutated rearrangements, with 71826 sequences including 7262 perfect matches to the inferred allele. The library had been generated from PBMC that had not been sorted for naïve B cells, resulting in the presence of reads of somatically hypermutated sequences in the data set, a fact that complicates the analysis. There was abundant variation in the CDR3 regions of the aligned sequences to an extent similar to that of other lambda germline alleles of this donor. One other alleles was present in the genotype. Haplotyping could not be performed. The inferred sequence includes 23 bases added to the 5’-end of incomplete reference allele IGLV2-14*03. There was support for the sequence being affirmed up to and including nucleotide 337, in line with IARC policy not to infer bases substantially affected by trimming during the rearrangement process, as a Level 1 sequence, with two additional 3’ nucleotide being likely present in the sequence. This is indicated in IARC and OGRDB publications by two dots at the end of the sequence. A genomic sequence (MK587524) has following the inference been cloned from the genotype of the donor that confirms the inference. It also confirms that bases 338-339 that cannot be inferred with confidence of the gene are T and C, respectively.