Submission ID | S00028 |
Submission Date | 2019-07-30 00:00:00 |
Submission Status | reviewing |
Submitter | Néstor Vázquez Bernat |
Submitter Address | Karolinska Institutet SE-171 77 Stockholm |
Species | Homo sapiens |
Ethnicity | UN |
Sequence | Subject | Genotype | Published | |
---|---|---|---|---|
IGHV3-20*01_S2478 | A007 | A007 VH | ||
IGHV3-7*02_A318G | A007 | A007 VH | A00006 | |
IGLV2-14*04x | A007 | A007 VL |
Repository | ENA |
Accession Number | PRJEB30386 |
Project/Study Title | High-quality immunoglobulin library preparation for germline inference and repertoire expression analysis. |
Dataset URL | https://www.ebi.ac.uk/ena/data/view/PRJEB30386 |
MiAIRR Compliant? | No |
MiAIRR URL | |
Sequencing Platform | Illumina MiSeq |
Read Length | 300nt |
Primers Overlapping? | No |
PubMed ID | Title | Authors |
---|---|---|
31024532 | High-Quality Library Preparation for NGS-Based Immunoglobulin Germline Gene Inference and Repertoire Expression Analysis. | Vázquez Bernat N, Corcoran M, Hardt U, Kaduk M, Phad GE, Martin M, Karlsson Hedestam GB |
IgM cDNA synthesis primer:
>Hu_IgM_MTPX
GGAGTTCAGACGTGTGCTCTTCCGATCTHHHHHACAHHHHHACAHHHHNHCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAAG
5’MTPX forward amplification set:
>Hu_VH_MTPX_1
CCTACACGACGCTCTTCCGATCTGGTGGCAGCAGTCACAGATGCCTACTC
>Hu_VH_MTPX_2
CCTACACGACGCTCTTCCGATCTGGTGGCAGCAGCCACAGGTGCCCACTC
>Hu_VH_MTPX_3
CCTACACGACGCTCTTCCGATCTGGTGGCAGCAGCTACAGGTGTCCAGTC
>Hu_VH_MTPX_4
CCTACACGACGCTCTTCCGATCTGGTGGSAGCAGCAACARGWGCCCACTC
>Hu_VH_MTPX_5
CCTACACGACGCTCTTCCGATCTGCTGGCTGTAGCTCCAGGTGCTCACTC
>Hu_VH_MTPX_6
CCTACACGACGCTCTTCCGATCTCCTGCTGCTGACCAYCCCTTCMTGGGTCTTGTC
>Hu_VH_MTPX_7
CCTACACGACGCTCTTCCGATCTCCTGCTACTGACTGTCCCGTCCTGGGTCTTATC
>Hu_VH_MTPX_8
CCTACACGACGCTCTTCCGATCTGGGTTTTCCTCGTTGCTCTTTTAAGAGGTGTCCAGTG
>Hu_VH_MTPX_9
CCTACACGACGCTCTTCCGATCTGGGTTTTCCTTGTTGCTATTTTAAAAGGTGTCCARTG
>Hu_VH_MTPX_10
CCTACACGACGCTCTTCCGATCTGGATTTTCCTTGCTGCTATTTTAAAAGGTGTCCAGTG
>Hu_VH_MTPX_11
CCTACACGACGCTCTTCCGATCTGGGTTTTCCTTKTKGCTATWTTAGAAGGTGTCCAGTG
>Hu_VH_MTPX_12
CCTACACGACGCTCTTCCGATCTGGTGGCRGCTCCCAGATGGGTCCTGTC
>Hu_VH_MTPX_13
CCTACACGACGCTCTTCCGATCTCTGGCTGTTCTCCAAGGAGTCTGTG
>Hu_VH_MTPX_14
CCTACACGACGCTCTTCCGATCTGGCCTCCCATGGGGTGTCCTGTC
>Hu_VH_MTPX_15
CCTACACGACGCTCTTCCGATCTGGTGGCAGCAGCAACAGGTGCCCACT
>Hu_VH_MTPX_16
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGAACTGGGGCTCCGCTGGGTTTTCC
>Hu_VH_MTPX_17
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGACTGCACCTGGAGGATCCTCCTC
>Hu_VH_MTPX_18
CACTCTTTCCCTACACGACGCTCTTCCGATCTTGGCTGAGCTGGGTTTYCCTTGTTGC
>Hu_VH_MTPX_19
CACTCTTTCCCTACACGACGCTCTTCCGATCTGGAGTTKGGGCTGMGCTGGGTTTTCC
>Hu_VH_MTPX_20
CACTCTTTCCCTACACGACGCTCTTCCGATCTGCACCTGTGGTTTTTCCTCCTGCTGGTG
>Hu_VH_MTPX_21
CACTCTTTCCCTACACGACGCTCTTCCGATCTCACCTGTGGTTCTTCCTCCTSCTGG
>Hu_VH_MTPX_22
CACTCTTTCCCTACACGACGCTCTTCCGATCTCCAGGATGGGGTCAACCGCCATCCTC
>Hu_VH_MTPX_23
CTCTTTCCCTACACGACGCTCTTCCGATCTCAGAGGACTCACCATGGAGTTTGGGCTGAG
>Hu_VH_MTPX_24
CCTACACGACGCTCTTCCGATCTGGACTCACCATGGAGTTGGGACTGAGC
>Hu_VH_MTPX_25
CCTACACGACGCTCTTCCGATCTGGGCTGAGCTGGCTTTTTCTTGTGGC
IgM cDNA synthesis primer:
>Hu_IgK_MTPX
GGAGTTCAGACGTGTGCTCTTCCGATCTHHHHHACAHHHHHACAHHHHNGGGATAGAAGTTATTCAGCAGGCACACAACAGAG
5’MTPX forward amplification set:
>Hu_VK_MTPX_1
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGTTGCCATCACAACTCATTGGGTTTCTG
>Hu_VK_MTPX_2
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGAARCCCCAGCGCAGCTTCTCTTCC
>Hu_VK_MTPX_3
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGAGGCTCCCTGCTCAGCTCTTGGGGCT
>Hu_VK_MTPX_4
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGAGGCTCCCTGCTCAGCTCCTGGGGCT
>Hu_VK_MTPX_5
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGACATGAGGGTCCCTGCTCAGC
>Hu_VK_MTPX_6
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGACATGAGRGTCCTCGCTCAGC
>Hu_VK_MTPX_7
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGAAGCCCCAGCACAGCTTCTTCTTCC
>Hu_VK_MTPX_8
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGAGGCTCCTTGCTCAGCTTCTGGGGCT
>Hu_VK_MTPX_9
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGAAGCCCCAGCTCAGCTTCTCTTCC
>Hu_VK_MTPX_10
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGACATGAGGGTCCCCGCTCAGC
>Hu_VK_MTPX_11
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGGGTCCCAGGTTCACCTCCTCAG
>Hu_VK_MTPX_12
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGTGTTGCAGACCCAGGTCTTCATTTC
>Hu_VK_MTPX_13
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGACATGAGGGTGCCCGCTCAGC
>Hu_VK_MTPX_14
CTCTTTCCCTACACGACGCTCTTCCGATCTCAGGAAGATGTYGCCATCACAACTCATTGG
>Hu_VK_MTPX_15
CACTCTTTCCCTACACGACGCTCTTCCGATCTCTCRCAATGAGGCTCCCTGCTCAGCTC
>Hu_VK_MTPX_16
CACTCTTTCCCTACACGACGCTCTTCCGATCTCCTGCTCAGCTCYTGGGGCTGCTAATGC
>Hu_VK_MTPX_17
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGACATGAGGGTGCCCGCTCAGCGCC
>Hu_VK_MTPX_18
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGACATGAGGGTSCCYGCTCAGCKCC
>Hu_VK_MTPX_19
CACTCTTTCCCTACACGACGCTCTTCCGATCTGCTCCTGGGGCTGCTAATGCTCTGG
>Hu_VK_MTPX_20
CACTCTTTCCCTACACGACGCTCTTCCGATCTGGGGCTCCTGCTGCTCTGGCTCC
>Hu_VK_MTPX_21
CACTCTTTCCCTACACGACGCTCTTCCGATCTGGACATGAGGGTCCCCGCTCAGCTCC
IgM cDNA synthesis primer:
>Hu_IgL_MTPX
GGAGTTCAGACGTGTGCTCTTCCGATCTHHHHHACAHHHHHACAHHHHTGGCTTGRAGCTCCTCAGAGGAGG
5’MTPX forward amplification set:
>Hu_VL_MTPX_1
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGGCTCCACTACTTCTCACCCTCC
>Hu_VL_MTPX_2
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGTCCCCTCTCTTCCTCACCCT
>Hu_VL_MTPX_3
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGGCTCTGCTCCTCCTCACCCT
>Hu_VL_MTPX_4
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGAYCCCTCTCCTGCTCCCCCTC
>Hu_VL_MTPX_5
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGGCTCTGCTGCTCCTCACTCT
>Hu_VL_MTPX_6
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCATGGATCCCTCTCTTCCTCGGCGTC
>Hu_VL_MTPX_7
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCATGGGCCACACTCCTGCTCCCACTC
>Hu_VL_MTPX_8
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGGTCTCCTTCTACCTACTGCCCT
>Hu_VL_MTPX_9
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACTCCTCTTCTTCTCTTGCTCCT
>Hu_VL_MTPX_10
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACTCCTCTCCTCCTCCTGYTCC
>Hu_VL_MTPX_11
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGAGTGTCCCCACCATGGCCTGGATGATGC
>Hu_VL_MTPX_12
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGGCTCCTCTGCTCCTCACCCTCC
>Hu_VL_MTPX_13
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGRCCDGCTTCCCTCTCCTCCTCACCCT
>Hu_VL_MTPX_14
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACCCCACTCCTCCTCCTCTTCC
>Hu_VL_MTPX_15
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGGCTCTGCTSCTCCTCASCCT
>Hu_VL_MTPX_16
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGATCCCTCTACTTCTCCCCCTC
>Hu_VL_MTPX_17
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACCSCTCTCCTCCTCRGCCTC
>Hu_VL_MTPX_18
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACTCTTCTCCTTCTCGTGCTCC
>Hu_VL_MTPX_19
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGTCTCCTCTCCTCCTCACTCT
>Hu_VL_MTPX_20
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGCCCTGGGCTCTGCTCCTCCTGACCCT
>Hu_VL_MTPX_21
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACCCCTCTCTGGCTCACTCTC
>Hu_VL_MTPX_22
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACCGCTCTCCTTCTGAGCCTC
>Hu_VL_MTPX_23
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCTTGGACCCCACTCCTCTTCCTCACC
>Hu_VL_MTPX_24
CTACACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACTCCTCTCTTTCTGTTCCTCC
>Hu_VL_MTPX_25
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACTCTTCTCCTTCTCGTG
>Hu_VL_MTPX_26
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACTCCTCTYCTYCTCYTG
>Hu_VL_MTPX_27
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGACCCCACTCCTCCTC
>Hu_VL_MTPX_28
CACTCTTTCCCTACACGACGCTCTTCCGATCTATGGCCTGGGTCTCCTTCTACCTACTGC
>Hu_VL_MTPX_29
CACTCTTTCCCTACACGACGCTCTTCCGATCTGCAGCATCGGAGGTGCCTCAGCCATG
>Hu_VL_MTPX_30
CACTCTTTCCCTACACGACGCTCTTCCGATCTGGCAGAACTCTGGGTGTCTCACCATG
>Hu_VL_MTPX_31
CACTCTTTCCCTACACGACGCTCTTCCGATCTGCAGCACTGGTGGTGCCTCAGCCATG
>Hu_VL_MTPX_32
CACTCTTTCCCTACACGACGCTCTTCCGATCTGGGCTCTGCTSCTCCTCACYCTCCT
>Hu_VL_MTPX_33
CACTCTTTCCCTACACGACGCTCTTCCGATCTGGGCTCTGCTCCTCCTGACCCTC
All the alleles have been genomically validated after reviewers request and their sequences have been submitted to genbank (MK587519-MK587529). Attached as well the plots of the genotype-statistics output for the three genotypes.
VL analysis and genotype updated 18 April 2020 with output from a new version of IgDiscover. 2-14*03 and 2-14*04 were subsequently classed as ‘novel’ alleles *o3x and *04x in order to fill the gaps in the current reference sequences