Assessed by IARC on November 18, 2019 during meeting 45.

The committee considered IGHV4-61*01_S7407 (C93T, C136G, A138C) of Genotype B12 of submission S00010, which was previously evaluated as IGHV4-61*01_S9594 of submission S00004 at Meeting 10. It was affirmed as a Level 0 sequence at that time.

The sequence was seen in 0.93% of all unmutated rearrangements, with 5928 sequences including 1572 perfect matches to the inferred allele. There was abundant variation in the CDR3 regions of the aligned sequences. Two other alleles were present in the genotype, with IGHV4-61*01 and IGHV4-61*02 also being present in the haplotype with this allele. Haplotype data is generally supportive of the inference, but in light of the complex structural variation seen in this particular individual, such a duplication is not an unlikely event. However based on this complexity and the similarities of IGHV4-4, IGHV4-59 and IGHV4-61 sequences, the sequence was affirmed as a Level 0 sequence. In line with IARC policy, the submitted sequence was recognized up to and including nucleotide 319. It is likely that one additional 3’ nucleotide is present in the sequence, and this is indicated in IARC and OGRDB publications by one dot at the end of the sequence.