Assessed by IARC on November 18, 2019 during meeting 45.

The committee considered IGHV1-69*14_S5279 (G163A) of Genotype B12 in submission S00010, which was previously evaluated as IGHV1-69*14_S3451 as part of submission S00004 at Meeting 8. It was affirmed as a Level 0 sequence at that time.

The sequence was seen in 2.29% of all unmutated rearrangements, with 11,849 sequences including 3889 perfect matches to the inferred allele. There was abundant variation in the CDR3 regions of the aligned sequences. Three other alleles were present in the genotype (none of which carries the allele-differentiating base A163), with IGHV1-69*13 also being present in the haplotype with this allele. In the earlier analysis (Meeting 8), an additional allele was reported. Haplotype data is supportive of the inference considering the existence of common duplications of the IGHV1-69 gene, but in light of the complex structural variation seen in this individual, the sequence was affirmed as a Level 0 sequence. In line with IARC policy, the submitted sequence was recognized up to and including nucleotide 319. It is likely that one additional 3’ nucleotide is present in the sequence, and this is indicated in IARC and OGRDB publications by one dot at the end of the sequence.