This sequence represents IGHV4-31*03 A70G in the IMGT numbering system. The committee noted a rearrangement frequency of 0.9%, with 812 alignments including 293 perfect matches to the inferred allele. There was abundant variation in the CDR3 regions of the aligned sequences. There was no alternative allele present in the genotype, but there are published reports (eg Gidoni et al 2019) of a deletion polymorphism involving the IGHV4-31 locus. All members of the committee agreed that the sequence should be accepted as a Level 1 sequence. Sequences have been submitted to SRA/ENA with acession numbers SRR8298743 and SRR8298742. The sequence was affirmed at level 1 at IARC Meeting 32 on March 1, 2019. In line with previous policy, the submitted sequence will be recognized up to and including nucleotide 319.

(April 12, 2019) A trailing “.” in the sequence indicates IARC’s opinion that the sequence is likely to contain additional 3’ nucleotides for which there is insufficient evidence to make an affirmation.