This inferred allele was assessed at IARC meeting 100 on June 8th, 2022. IGHV3-43D*04_G4A was inferred in subject S10 (VDJ-base: P1_I10_S1). The genotype also carried IGHV3-43*01 but no other allele of IGHV3-43D. The inference was supported by a relatively small number of sequences (104) and unmutated sequences (93), a low overall frequency in the unmutated population (0.3%) and a small but diverse set of unique CDR3s (89) in the unmutated sequence set. Its allelic ratio was 100%. Haplotyping based on allelic diversity in IGHJ6 was possible and the alleles distributed to one haplotype, a haplotype different from that associated with the allele IGHV3-43 (IGHV3-43D*04_G4A: 100:0; IGHV3-43*01: 0:100). Reads of IGHV3-43D*04_G4A were associated to the same haplotype as those of IGHV4-38-2, a common duplication combination. Reads of IGHV3-43D*04_G4A were associated to an upstream region similar to that of other alleles of IGHV3-43D (but not similar to alleles of IGHV3-43) (doi: 10.3389/fimmu.2021.730105). Altogether, the association of this allele to IGHV3-43D is reasonable. IARC affirms the sequence at level 1 up to and including base 321 in agreement with past practice. It is acknowledged that the allele most likely carries one additional base, typically A at base position 322. A trailing “.” indicates IARC’s opinion that the sequence is likely to contain one additional 3’-nucleotides for which there is insufficient evidence to make an affirmation. The allele is given the name IGHV3-43D*i02.

Additional information for this sequence was imported into OGRDB via bulk update with the following notes:
Sequence annotation is based on Genbank sample OW151136
VDJbase example haplotype: Y
Mapped by IARC haplotype