Information for this sequence was imported into OGRDB via bulk update with the following notes:
Sequence annotation is based on Genbank sample CP068256

The 5’ extension relative to IMGT IGLV2-14*01 is supported by IARC analysis of Submission S00028, to which the following notes refer:

Assessed by IARC on July 7, 2020 during meeting 57. The committee considered IGLV2-14*04x (a 5’ extension of IGLV2-14*04) of submission S00028.

The sequence was seen in 6.88% of all unmutated rearrangements, with 21083 sequences including 8224 perfect matches to the inferred allele. The library had been generated from PBMC that had not been sorted for naïve B cells, resulting in the presence of reads of somatically hypermutated sequences in the data set, a fact that complicates the analysis. There was abundant variation in the CDR3 regions of the aligned sequences to an extent similar to that of other lambda germline alleles of this donor. One other alleles was present in the genotype. Haplotyping could not be performed. The inferred sequence includes 23 bases added to the 5’-end of incomplete reference allele IGLV2-14*04. There was support for the sequence being affirmed up to and including nucleotide 337, in line with IARC policy not to infer bases substantially affected by trimming dring the rearrangement process, as a Level 1 sequence, with two additional 3’ nucleotide being likely present in the sequence.