Submission Details

Submission IDS00043
Submission Date2024-02-06 00:00:00
Submission Statusreviewing
SubmitterWilliam Lees
Submitter AddressBirkbeck College, University of London, Malet Street, London
SpeciesHomo sapiens
EthnicityUN

Inferred Sequences

The inferred novel alleles from each genotype that are submitted for review. This table lists all inferences put forward by the submitter. Where IARC has affirmed a sequence based on an inference, the corresponding sequence record will be listed in the Published column. Inferences for which no published sequence is shown have not been affirmed.

SequenceSubjectGenotypePublished
 IGKV3-15*01_A117GS24P1_I24_S1_ogrdb_reportA03368

Genotypes

Each genotype that has been inferred, along with the descriptive name of the inference tool and settings that were used.

Genotype NameSubject IDLocusSequence TypeGenotype FilenameTool/Setting Name
 P1_I24_S1_ogrdb_reportS24IGKVP1_I24_S1_ogrdb_report.csvTIgGER

Acknowledgements

Individuals who should be acknowledged as contributing to the inferences listed in this submission.

No Items

Repertoire Details

Details of the repertoire from which the inferences are based. This corresponds, for example, to an NIH Project or an ENA study.

RepositoryNCBI SRA
Accession NumberPRJEB26509
Project/Study TitleIllumina MiSeq paired end sequencing
Dataset URLhttps://www.ncbi.nlm.nih.gov/sra/?term=PRJEB26509
MiAIRR Compliant?No
MiAIRR URL
Sequencing PlatformMiSeq 2x300
Read Length300x300
Primers Overlapping?No

Repertoire Publications

Publications associated with this study.

PubMed IDTitleAuthors
30733445Mosaic deletion patterns of the human antibody heavy chain gene locus shown by Bayesian haplotyping.Gidoni M, Snir O, Peres A, Polak P, Lindeman I, Mikocziova I, Sarna VK, Lundin KEA, Clouser C, Vigneault F, Collins AM, Sollid LM, Yaari G

Sequences of the PCR primers used in the study.

3' Primers

As the protocol used was 5’ RACE, there is only one primer set.

Primer NamePrimer Sequence
Human-IGHMGAATTCTCACAGGAGACGAGG
Human-IGHDTGTCTGCACCCTGATATGATGG
Human-IGHAGGGTGCTGYMGAGGCTCAG
Human-IGHETTGCAGCAGCGGGTCAAGG
Human-IGHGCCAGGGGGAAGACSGATG
Human-IGKGACAGATGGTGCAGCCACAG
Human-IGLAGGGYGGGAACAGAGTGAC

Inference Tools and Settings

Details of the inference tools and settings used to infer novel alleles. Each combination of tool and setting is listed here, and provided with a descriptive name.

Tool/Settings NameTool NameTool Version
 TIgGERTIgGER1.0.0

Notes

IGKV3-15*01_a117g submission created from VDJbase

Supplementary Files
 P1_I24_S1_ogrdb_plots.pdf